教員紹介

神崎 真哉
  • 神崎 真哉
  • 准教授
所属 農学部 農業生産科学科
農学研究科
附属農場
学位 博士(農学)
専門 果樹園芸学
コメント 果物に関する研究をしています。特にカキやマンゴーの育種を効率よく進める方法をDNA解析等を利用して開発しています。その他の温帯果樹、熱帯果樹の栽培に関する研究も行っています。
リサーチマップリンク https://researchmap.jp/read0057430

研究活動情報

研究分野

  • 環境・農学, 園芸科学

研究キーワード

果樹園芸学

論文

  1. Analysis of genetic diversity of lychee (Litchi chinensis Sonn.) and wild forest relatives in the Sapindaceae from Vietnam using microsatellites , Hoa Tran, Shinya Kanzaki, Ludwig Triest, Inaki Hormaza, Na Jong Kuk, Ray Ming, Jean Bousquet, Damase Khasa, Patrick Van Damme , Genetic Resources and Crop Evolution , 66 , 1653-1669 , 2019年12月1日
  2. Isolation of UDP:flavonoid 3-<i>O</i>-glycosyltransferase (UFGT)-like Genes and Expression Analysis of Genes Associated with Anthocyanin Accumulation in Mango 'Irwin' skin , Kanzaki Shinya, Kamikawa Shiori, Ichihi Asuka, Tanaka Yuta, Shimizu Kosuke, Koeda Sota, Utsunomiya Naoki , The Horticulture Journal , 88 , 4 , 435-443 , 2019年10月 , 査読有り
  3. Pepper yellow leaf curl Aceh virus: a novel bipartite begomovirus isolated from chili pepper, tomato, and tobacco plants in Indonesia , Kesumawati Elly, Okabe Shoko, Homma Kanami, Fujiwara Ikuya, Zakaria Sabaruddin, Kanzaki Shinya, Koeda Sota , ARCHIVES OF VIROLOGY , 164 , 9 , 2379-2383 , 2019年9月 , 査読有り
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講演・口頭発表等

  1. 異なる非辛味化因子を有するトウガラシ(Capsicum chinense)品種における果実の揮発性成分分析 , 蓮真海, 尾崎早也佳, 大野公輝, 木下万智子, 富研一, 田中義行, 神崎真哉, 神崎真哉, 小枝壮太, 小枝壮太 , 園芸学研究 別冊 , 2018年9月22日
  2. 新たに見出した組換え型PepYLCIVの感染性クローンを用いたベンサミアナタバコへの接種 , 岡部祥子, KESUMAWATI Elly, ZAKARIA Sabaruddin, 神崎真哉, 小枝壮太 , 園芸学研究 別冊 , 2018年9月22日
  3. Ty‐3a抵抗性トマト品種におけるTYLCKaVの増殖およびウイルスゲノムのメチル化 , 藤原郁也, KESUMAWATI Elly, ZAKARIA Sabaruddin, 神崎真哉, 神崎真哉, 小枝壮太, 小枝壮太 , 園芸学研究 別冊 , 2018年9月22日
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MISC

  1. Development of EST-SSR markers derived from diospyros kaki , T. Tsujimoto, T. Akagi, K. Yonemori, S. Kanzaki , Acta Horticulturae , 996 , 133 , 138 , 2013年7月26日 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84880441457&origin=inward
    概要:For genetic analysis of persimmon (Diospyros kaki) and its relatives, we investigated the development of SSR markers with expression sequence tags (ESTs). Of 9475 EST data from the NCBI database which had isolated from D. kaki, 746 sequences contained SSRs which had more than four (tetranucleotide) or five (dinucleotide and trinucleotide) repeats. Tested on two species, D. kaki and D. lotus (a diploid relative of D. kaki), of primer pairs based on 65 SSR-containing EST sequences, 52 primer pairs resulted in proper PCR products. Some of these candidate EST-SSR markers showed polymorphism among cultivars and seedlings of D. kaki and D. lotus. Among tested seven clones of D. lotus, 32 of 52 candidate EST-SSR markers showed polymorphism. For molecular phylogenetic analysis, linkage mapping and whole genome mapping of Diospyros species, EST-SSR markers appear to be as useful as genomic SSR markers. In addition, development of EST-SSR markers seems to be easy and cost-effective when compared with genomic SSR markers.
  2. Construction of a reliable PCR marker for selecting pollination constant and non-astringent (PCNA) type offspring among breeding population of persimmon (Diospyros kaki Thunb.) , K. Yonemori, T. Akagi, S. Kanzaki , Acta Horticulturae , 839 , 625 , 629 , 2009年12月1日 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=75649102794&origin=inward
    概要:Pollination constant and non-astringent (PCNA) type persimmons, which lose their astringency naturally on the tree, are the most desirable for fresh fruit consumption. The trait of natural astringency-loss in PCNA type is recessive and controlled by a single locus, known as AST locus. Because PCNA type cultivars are of recent origin and exist only in a small number of cultivars, inbreeding depression resulting from repeated crosses among very few cultivars and/or selections is a major challenge facing the current breeding of new PCNA type cultivars. One solution to overcome this inbreeding depression is to include non-PCNA cultivars in the breeding project to extend the genetic pool by utilizing the wide diversity found in non-PCNA cultivars. However, to date, F1 populations resulting from crosses between PCNA and non-PCNA type cultivars/selections have yielded only non-PCNA offspring. To obtain PCNA offspring, F1 progeny needs to be backcrossed to PCNA type cultivars/selections. As persimmon is hexaploid, the obtainable rate of PCNA offspring in a backcross population is only 10-15%. Although we identified AFLP and RFLP markers tightly linked to the trait of natural astringency-loss in persimmon, these markers could not distinguish some non-PCNA progenies in other breeding populations. Previously, we demonstrated that RFLP markers derived from fosmid clones of D. lotus, diploid relatives of D. kaki, could be used to distinguish between PCNA and non-PCNA types of D. kaki, and established the possibility of extending this approach to PCR based markers. In this study, we describe the development of PCR-based markers derived from fosmid clones of D. kaki and D. lotus, and confirm their effectiveness across diverse progenies to select PCNA type.
  3. Phylogeny and cultivar development of Diospyros kaki: A survey based on molecular analyses , K. Yonemori, S. Kanzaki, C. Honsho, T. Akagi, D. E. Parfitt , Advances in Horticultural Science , 22 , 261 , 268 , 2008年12月9日 , https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57149106746&origin=inward
    概要:The relationships of Diospyros kaki to 14 Diospyros species, including D. glandulosa which was assumed as a progenitor of D. kaki by Ng (Malaysian Forester 41: 43-50), were studied by a combined data set of the sequences for ITS region and matK gene. Maximum parsimony, maximum likelihood, and neighbor joining analyses revealed that D. oleifera, a species native to China, was much closer to D. kaki than D. glandulosa. However, both D. oleifera and D. glandulosa were more closely related to D. kaki, compared with other species analyzed, indicating that these two diploid species might be involved in the origin of D. kaki, most of which is hexaploid. In addition, as for D. kaki, 102 Japanese, 20 Korean, and 24 Chinese cultivars were studied for their genetic relationships by comparing 496 AFLP markers to investigate cultivar development of D. kaki in Asia. An unrooted cladogram constructed by neighbor joining analysis indicated independent cultivar development in China, Korea, and Japan. Furthermore, pollination constant non-astringent (PCNA) type cultivars of Japanese origin were clearly separated from non-PCNA type cultivars in the cladogram, also indicating independent development of PCNA cultivars in Japan. Cultivar development of D. kaki in Asia is discussed further in the work, with focus on the astringency trait.
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受賞

  1. 2011年3月, 園芸学会, 年間優秀論文賞, SCAR markers for practical application of marker-assisted selection in persimmon (Diospyros kaki) breeding.
  2. 2010年3月, 園芸学会, 奨励賞, 完全甘ガキ個体選抜のための分子マーカーの構築とそれを利用したカキの遺伝様式の解析
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競争的資金

  1. 文部科学省, 科学研究費補助金(基盤研究(C)), マンゴーの花成誘導を制御する分子機構の解明
  2. 文部科学省, 科学研究費補助金(基盤研究(C)), マンゴーの交雑特性の解明と育種基盤の確立
  3. 文部科学省, 科学研究費補助金(若手研究(B)), 熱帯果樹の果実着色機構と高温下でのアントシアニン合成
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