Researchers

TAKEDA Toru

TAKEDA Toru
Associate Professor
Faculty Department of Advanced Bioscience / Graduate School of Agriculture
Researchmap https://researchmap.jp/read0181514

Education and Career

Education

  • - 1989 , Kindai University, Faculty of Agriculture,
  • - 1989 , Kinki University, Faculty of Agriculture,
  • - 1991 , Kindai University, Graduate School of Agriculture,
  • - 1991 , Kinki University, Graduate School, Division of Agriculture,

Research Activities

Research Areas

  • Life sciences, Plant nutrition, soil science

Research Interests

glutathione, tellurium, selenium, reactive oxygen species, phytoremediation, trace elements, bio metal, Plant Metabolic Biochemistry

Published Papers

  1. Selenium is involved in the detoxification of methylglyoxal in Arabidopsis seedlings.
    TAKEDA Toru
    Biomedical Research on Trace Elements  27  (1)  , 125-131, Jun. 2016  , Refereed
  2. Antioxidant responses of selenium- enriched broccoli sprout (Brassica oleracea) to paraquat exposure.
    TAKEDA Toru; KONDO Ken; UEDA Kazunori; IIDA Akira
    Biomedical Research on Trace Elements  27  (1)  , 8-14, Apr. 2016  , Refereed
  3. Post-translational activation of non-selenium glutathione peroxidase of Chlamydomonas reinhardtii by specific incorporation of selenium
    Toru Takeda
    Biochemistry and Biophysics Reports  4  , 39-43, 1, Dec. 2015  , Refereed

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Books etc

  1. The H2O2-scavenging system and tolerance system to H2O2 in algae. , "Frontiers of Reactive Oxygen Spacies in Biology and Madicine", Excerpta Medica , 1994

Conference Activities & Talks

  1. 植物におけるレアアースの新規生理機能の解明 , 松田 旭生; 飯田 諒; 西尾 直輝; 小嶋 えみり; 高貝 俊生; 武田 徹 , メタルバイオサイエンス研究会2020・生命金属に関する合同年会 , 7, Nov. 2020
  2. 植物由来セレン化タンパク質のセレン結合機構の解明 , 高貝 俊生; 小嵜 光夏; 武田 徹 , メタルバイオサイエンス研究会2020・生命金属に関する合同年会 , 7, Nov. 2020
  3. 毒性元素セレン・テルルが植物の抗酸化力に及ぼす影響 , 高貝俊生; 山本佳乃子; 塚田 良; 武田 徹 , 2019年度日本土壌肥料学会関西支部会 , 5, Dec. 2019

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MISC

  1. Beneficial effects of selenium as a micronutrient in Arabidopsis seedlings , 武田 徹 , Selenium 2010 , P095 , Jun. 2010
    Summary:セレン非蓄積植物であるシロイヌナズナを微量のセレン（1 ?Mセレン酸ナトリウム）を含む培地で生育させると、播種後17日目までの実生の段階で、生育の有意な促進効果が認められた。この植物における解糖系酵素のNAD依存グリセルアルデヒド-3-リン酸デヒドロゲナーゼ（NAD-GAPDH）の活性、ATP生成、および呼吸活性が有意に増加していた。セレン存在下で生育させた植物体より精製したNAD-GAPDHを質量分析したところ、活性中心に相当する155番目のシステイン残基にセレンが結合していることが明らかになった。
  2. Values of water-soluble vitamins in blood and urine of Japanese young men and women consuming a semi-purified diet based on the Japanese dietary reference intakes , Katsumi Shibata; Tsutomu Fukuwatari; Mari Ohta; Hidemi Okamoto; Toshiaki Watanabe; Tooru Fukui; Mamoru Nishimuta; Masayuki Totani; Mieko Kimura; Nobuko Ohishi; Mieko Nakashima; Fumio Watanabe; Emi Miyamoto; Shigeru Shigeoka; Tooru Takeda; Megumi Murakami; Hiroshi Ihara; Naotaka Hashizume , Journal of Nutritional Science and Vitaminology , 51 , 5 , 319 , 328 , 2005
    Summary:We investigated the levels of water-soluble vitamins except for vitamin B6 in the blood and urine of Japanese college male (n=10) and female (n=10) students. They consumed for 7 d a semi-purified diet based on Japanese Dietary Reference Intakes to assess the Recommended Dietary Allowances (RDA) for water-soluble vitamins and to present some new normal values for blood and urine levels of water-soluble vitamins in Japanese. The blood and the 24-h urine samples were collected on the last day of the experiment and measured. The values of total vitamin B1 in whole blood, total vitamin B 2 in whole blood, total cyanocobalamin in serum, total nicotinamide in whole blood, total pantothenic acid in whole blood, total folates in serum, total biotin in serum, and ascorbic acid in plasma were 104±17 pmol/mL (mean±SD), 216±25 pmol/mL, 0.34±0.05 pmol/mL, 59.1±5.0 nmol/mL, 2.45±0.37 nmol/mL, 15.6±4.6 pmol/mL, 8.3±0.5 pmol/mL, and 62±10 nmol/mL, respectively, in males, and 90±23, 234±18, 0.67±0.20, 61.9±6.0, 2.48±0.30, 30.2± 8.6, 8.4±0.3, and 67±14, respectively, in females. There was a significant difference in the values of cyanocobalamin and total folates between men and women. The urinary excretion of vitamin B1, vitamin B2, cyanocobalamin, sum of the catabolic metabolites of nicotinamide, pantothenic acid, folates, biotin, and ascorbic acid were 665±114 nmol/d, 562± 325 nmol/d, 93±31 pmol/d, 84±26 μmol/d, 9.3±2.3 μmol/d, 19.4±2.8 nmol/d, 83±18 pmol/d, and 148±51 μmol/d, respectively, in males, and 495±212, 580±146, 145±49, 83±19, 16.9±1.3, 22.7±2.7, 83±23, and 140±51, respectively, in females. There was a significant difference in the urinary excretion of cyanocobalamin, pantothenic acid and total folates between men and women. These values will be useful for the nutritional assessment of water-soluble vitamins for Japanese, although the examination period was short. In future, an experiment with various age groups and re-evaluation by repeated experiments will provide more accurate values.
  3. Induction and functional analysis of two reduced nicotinamide adenine dinucleotide phosphate-dependent glutathione peroxidase-like proteins in Synechocystis PCC 6803 during the progression of oxidative stress , A Gaber; K Yoshimura; M Tamoi; T Takeda; Y Nakano; S Shigeoka , PLANT PHYSIOLOGY , 136 , 1 , 2855 , 2861 , Sep. 2004
    Summary:Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed by high light intensity, high salinity, or application of methylviologen or t-butyl hydroperoxide in the wild-type and GPX-2 knock-out mutant (gpx-2Delta) cells. To examine the ability of GPX-1, GPX-2, and thioredoxin peroxidase to scavenge lipid hydroperoxide in vivo, we measured the photosynthetic evolution of O-2 and the level of lipid peroxidation in the wild-type and each type of mutant cell after the application of t-butyl hydroperoxide or H2O2. The data reported here indicate that GPX-1 and GPX-2 are essential for the removal of lipid hydroperoxides under normal and stress conditions, leading to the protection of membrane integrity.

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Research Grants & Projects

  1. 日本学術振興会, 科学研究費 基盤研究(C), 環境浄化をめざした植物におけるセレン・テルルの代謝および集積機構の解明
  2. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Novel physiological function of selenium and selenium-dependent regulatory factor in Arabidopsis thaliana. , Kinki University
  3. 日本学術振興会, 科学研究費助成事業, 環境ストレス耐性植物の分子育種のための多重遺伝子導入の確立 , 近畿大学

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