Researchers

NAKASONE Kaoru

NAKASONE Kaoru
Professor
Faculty Department of Biotechnology and Chemistry / Graduate School of System Engineering
Researchmap https://researchmap.jp/nakasonek

Education and Career

Education

  • 1983/04 - 1987/03 , University of the Ryukyus, Faculty of Science,
  • 1988/04 - 1990/03 , University of the Ryukyus, 大学院理学研究科修士課程,
  • 1990/04 - 1994/03 , University of the Ryukyus, Graduate School of Medicine,

Academic & Professional Experience

  • Apr. 1994 - Mar. 2001 , The DEEP STAR Group, Japan Marine Science and Technology Center Research Associate
  • Apr. 1987 - Mar. 1988 , 東京大学 海洋研究所 海洋生化学部門 研究生

Research Activities

Research Areas

  • Life sciences, Applied biochemistry
  • Life sciences, Functional biochemistry
  • Life sciences, Genetics

Research Interests

ゲノム解析, 植物病原菌, 黒麹菌, 高度好塩性アーキア, 深海微生物, 極限環境微生物, Genomics, Extremely halophilic archaeon, Deep-sea microorganism

Published Papers

  1. Draft Genome Sequence of Saccharomyces cerevisiae DJJ01, Isolated from Dojoji Temple in Gobo, Wakayama, Japan
    Shinnosuke Okuhama; Kaoru Nakasone; Kazuki Yamanaka; Chiho Miyazaki; Tsumugi Nakamoto; Yuki Nakashima; Masataka Kusube
    Microbiology Resource Announcements  11  (8)  18, Aug. 2022  , Refereed
  2. Sterilization Effects of HO2/O2-Radicals Produced by H2O-O2 Plasma
    Kosei Satahira; Kaoru Nakasone; Tatsuhiko Ihara
    Journal of Photopolymer Science and Technology  29  (3)  , 433-438, Jun. 2016  , Refereed
  3. Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica
    Rie Yatsunami; Ai Ando; Ying Yang; Shinichi Takaichi; Masahiro Kohno; Yuriko Matsumura; Hiroshi Ikeda; Toshiaki Fukui; Kaoru Nakasone; Nobuyuki Fujita; Mitsuo Sekine; Tomonori Takashina; Satoshi Nakamura
    FRONTIERS IN MICROBIOLOGY  5  (5)  , 100-104, Mar. 2014  , Refereed

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Conference Activities & Talks

  1. 実験，実習をベースとした生物工学/化学の導入・動機づけ教育への取り組み－平成15年度文部科学省サイエンス・パートナーシップ・プログラム報告－ , 白石 浩平; 鈴木 克之; 仲宗根 薫; 岡田 芳治 , 平成17年度（第53回）日本工学教育協会 , Sep. 2005
  2. Structural analysis of the nitrogen fixation-related genes from Paenibacillus ozotofixans , 鈴木 克之; 坂本秀樹; 西紋郁; 仲宗根 薫; 寺岡孝敏 , 平成14年度日本生物工学会大会（大阪） , Oct. 2002
  3. 深海由来好冷好圧性細菌 Shewanella violacea のリボソーム関連遺伝子のゲノム解析 , 仲宗根 薫; 吉岡孝文; 木村明日香 , 2002 年度日本放線菌学会大会 （つくば） , May. 2002

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MISC

  1. Newly identified sigma factors of RNA Polymerase from deep-sea bacterium Shewanella violacea , NAKASONE Kaoru; KATO Chiaki; KAWANO Hiroaki , 近畿大学工学部研究報告 , 44 , 15 , 20 , 2010
    Summary:Two genes for alternative a factors, σ^<E2> and σ^<E3>, classified in the extracytoplasmic function a family for RNA polymerases, were identified in the deep-sea piezophilic bacterium Shewanella violacea DSS12. Amino acid alignments revealed that the domains for transcriptional functions are were comparatively conserved compared with E. coli σ^E in both proteins. Core-binding analysis suggests suggested that both proteins function as a factors in this piezophilic bacterium.
  2. Structure analysis of BasS and BasR in rice seedling blight pathogen Burkholderia plantarii , Yamano Yumiko; Nakasone Kaoru , 近畿大学工学部研究報告 , 43 , 31 , 35 , 2009
    Summary:Burkholderia plantani is a plant pathogen causing rice seedling blight. It produces tropolone, the disease causing substance. To elucidate and understand molecular mechanisms of the pathogenesis, we analyzed the genome of B. plantarii. In this study, we focused on two component system, in prokaryotic signal transduction. This system is basically composed of a histidine kinase (HK, sensor) residing in the inner membrane and a cognate response regulator (RR) in the cytoplasm. Here, BasS, a histidine kinase (HK, sensor) and BasR, a response regulator (RR) in this strain, were structurally analysed, showing these are tandemely ordered. Phylogenetic analysis of these prodcuts were also analysed. Construction of plasmids for gene disruption of these genes, basS and basR was also carried out, in terms of future study approach in reverse genetics. Obtaining mutants by transformation will be useful for understanding the relationships between pathogenesis and function of these genes.
  3. Amino acid sequence diversities in TBP, TATA binding protein, of extremely halophilic archaeon Haloarcula japonica strain TR-1 , Matsumi Hironari; Nakasone Kaoru , 近畿大学工学部研究報告 , 42 , 17 , 20 , 2008
    Summary:The TATA-box binding protein (TBP) is a basal transcription factor involved in transcription initiation in Eukarya and Archaea. Through exhaustive analyses of the whole geneme of extremely halophilic archaeon, Haloarcula japonica strain TR-1, six TBP genes were found and structurally analyzed. These TBPs were designated as TBP1, TBP2, TBP3, TBP4, TBP5 and TBP6, respectively and these TBPs were diverged from other archaeal TBPs that have been known. TBP1 gene was found to encode a polypeptide consisting of 182 amino acid residues, showing 35.0％ identity to that of H. marismortui. TBP2 gene was found to encode a polypeptide consisting of 182 amino acid residues, showing 36.5％ identity to that of H. marismortui. TBP3 gene was found to encode a polypeptide consisting of 186 amino acid residues, showing 100％ identity to that of H. marismortui. TBP4 gene was found to encode a polypeptide consisting of 185 amino acid residues, showing 42.9％ identity to that of H. marismortui. TBP5 gene was found to encode a polypeptide consisting of 182 amino acid residues, showing 35.4％ identity to that of H. marismortui. TBP6 gene was found to encode a polypeptide consisting of 182 amino acid residues, showing 46.1％ identity to that of H. marismortui. By phylogenetic analyses of these six proteins, TBP3 is conserved in amino acid sequence with other archaeal strains including methanogens and thermophiles, It may suggest that the TBP3 is core TBP function in transcriptional initiation such as housekeeping genes. Six histidine-tagged version of the H. japonica TBPs were produced in Escherichia coli in a denature conditions after construction of overexpression plasmids and purified by means of Ni-chelating chromatography.

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Awards & Honors

  1. Dec. 2003, 極限環境微生物学会, 奨励賞

Research Grants & Projects

  1. （独）日本学術振興会, 2023年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI, 高圧力で"調べ隊"：深海の高圧力に耐える生物を見つけよう！ , 近畿大学
  2. 公益財団法人サタケ技術振興財団, イネ苗立枯れ病を防除するための、環境に優しい新規な物質の探索
  3. （独）日本学術振興会, 2022年度 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI, 深海微生物探検隊；-高圧力に耐える微生物の不思議ｰ , 近畿大学

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