Researchers

KURASHITA Hazuki

KURASHITA Hazuki: Research associate

Faculty	Department of Biotechnological Science
Researchmap	https://researchmap.jp/hkurashita

Education and Career

Education

2018/04 - 2020/03 , National Institute of Technology, Miyakonojo College, 物質工学科物質工学専攻,
2020/04 - 2025/03 , Nagaoka University of Technology, 技術科学イノベーション専攻,

Academic & Professional Experience

2025 - Today , Kindai University Faculty of Biology-Oriented Science and Technology Department of Biotechnological Science 助教

Research Activities

Research Areas

Other, Other

Research Interests

環境微生物

Published Papers

Development of an internal two-stage upflow anaerobic reactor integrating biostimulation strategies to enhance the degradation of aromatic compounds in wastewater from purified terephthalic acid and dimethyl terephthalate manufacturing processes.
Kyohei Kuroda; Maho Takai; Takeo Sekiguchi; Tomoya Ikarashi; Hazuki Kurashita; Meri Nakajima; Masaru K Nobu; Masashi Hatamoto; Takashi Yamaguchi; Yuki Nakaya; Hisashi Satoh; Masahito Yamauchi; Masayoshi Yamada; Takashi Narihiro
Water research 258 , 121762-121762, 1, Jul. 2024
Corallococcus caeni sp. nov., a novel myxobacterium isolated from activated sludge.
Shun Tomita; Ryosuke Nakai; Kyohei Kuroda; Hazuki Kurashita; Masashi Hatamoto; Takashi Yamaguchi; Takashi Narihiro
Archives of microbiology 206 (7) , 317-317, 21, Jun. 2024
Draft genome sequences of Corallococcus strains KH5-1 and NO1, isolated from activated sludge.
Shun Tomita; Ryosuke Nakai; Kyohei Kuroda; Hazuki Kurashita; Masashi Hatamoto; Takashi Yamaguchi; Takashi Narihiro
Microbiology resource announcements 13 (2) , e0110423-, 15, Feb. 2024

Estimation of potential metabolic functions and life cycles of phylum Myxococcota bacteria ubiquitously found in activated sludge systems , 蔵下はづき; 幡本将史; 山口隆司; 富田駿; 成廣隆; 黒田恭平 , 日本水環境学会年会講演集 , 59th , 2025
Comprehensive Insights into Potential Metabolic Functions of Myxococcota in Activated Sludge Systems , Kurashita Hazuki; Kurashita Hazuki; Hatamoto Masashi; Tomita Shun; Yamaguchi Takashi; Narihiro Takashi; Narihiro Takashi; Kuroda Kyohei; Kuroda Kyohei , Microbes and Environments , 39 , 4 , 2024
Summary:Myxobacteria, belonging to the phylum Myxococcota, are ubiquitous in soil, marine, and other environments. A recent metagenomic sequencing ana-lysis showed that Myxococcota are predominant in activated sludge systems; however, their metabolic traits remain unclear. In the present study, we exami-ned the potential biological functions of 46 metagenomic bins of Myxococcota reconstructed from activated sludge samples from four municipal sewage treatment plants. The results obtained showed that most Myxococcota bins had an almost complete set of genes associated with glycolysis and the TCA cycle. The Palsa-1104 and Polyangiales bins contained the glycoside hydrolase GH5 and peptidase M23, which are presumably involved in lysis of the cell wall and cellular cytoplasm, suggesting that some Myxococcota from activated sludge prey on other microorganisms. The cell contact-dependent predatory functions of Myxococcus xanthus are conserved in the family Myxococcaceae, but not in other families. Two bins belonging to Palsa-1104 had phototrophic gene clusters, indicating the potential for heterotrophic and autotrophic metabolism by these microbes. In assessments of the social behavior of Myxococcota in activated sludge, the FruA gene and C-signal gene, which are involved in the regulation of fruiting body formation, were lacking in Myxococcota bins, suggesting their inability to form fruiting bodies. In addition, multiple bins of Myxococcota had novel secondary metabolite biosynthesis gene clusters that may be used for the predation of other bacteria in activated sludge. Our metagenome-based ana-lyses provide novel insights into the microbial interactions associated with Myxococcota in activated sludge ecosystems.
Attempt at isolation of uncultured Myxococcota predominant in activated sludge and elucidation of the ecological functions. , 蔵下はづき; 幡本将史; 山口隆司; 富田駿; 成廣隆; 黒田恭平 , 日本水環境学会年会講演集 , 58th , 2024