Researchers
- KATO Yoko
- Professor
Faculty | Department of Advanced Bioscience / Graduate School of Agriculture |
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Researchmap | https://researchmap.jp/AnimalReprod |
Education and Career
Academic & Professional Experience
- 2009 - Today , KINDAI University Agriculture Professor
Research Activities
Research Areas
- Life sciences, Laboratory animal science
- Life sciences, Animal production science
Published Papers
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Rescue of oocytes recovered from postmortem mouse ovaries.
Nagi Fujii; Yuta Nakata; Yoko Kato
Experimental animals 15, Aug. 2022 , Refereed -
Gamete and Embryo Technology: Cloning
Yoko Kato
Encyclopedia of Dairy Sciences (Third edition) , 868-873, 2022 , Refereed -
Effect of phytohemagglutinin (PHA) on nuclear transfer pig embryos in vitro
Ndubuisi Machebe; Soichiro Shimizu; Masaki Hata; Kazuki Ohata; Tetsuya Tani; Yoko Kato
Journal of Mammalian Ova Research 36 , 33-43, Apr. 2019 , Refereed
Conference Activities & Talks
- p53 遺伝子がもたらすエピブラスト様細胞への誘導機構 , 青木雪来; 加藤容子; 岡村大治 , 第39回日本受精着床学会 , 15, Jul. 2021
- 家畜繁殖学研究の最先端 ~基礎から臨床へ~ , 加藤容子 , 第39回日本受精着床学会 , 15, Jul. 2021
- GV期核置換卵の生存性向上に関わる要因 , 真柄怜央; 加藤容子 , 第62回日本卵子学会 , 29, May. 2021
MISC
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〈原著〉FGF9 の添加がブタ体外成熟・体外発生能に及ぼす影響
, アニエクェ, ・チオマ・ジェーン; 前山, 怜; 真柄, 怜央; 青木, 雪来; 東野, 愛理; 加藤, 容子 , 近畿大学農学部紀要 = MEMOIRS OF THE FACULTY OF AGRICULTURE OF KINDAI UNIVERSITY , 55 , 15 , 24 , 31, Mar. 2022
Summary:type:Departmental Bulletin Paper [Synopsis] Fibroblast growth factor 9 (FGF9) plays a key role in the regulation of cell differentiation, cell proliferation and embryonic development. It is also a survival factor for various cell types and interacts with two FGF receptors, FGFR2 and FGFR3. Furthermore, the absence of specific thecal-and oocyte-derived products during in vitro maturation (IVM) is suggested to be one reason for the reductions in the competency of oocytes used in assisted reproductive techniques. FGF9, as with some FGFs shown to promote oocyte competence and embryo development in several species is localize largely in theca cells, somewhat in the oocyte and its receptors in oocytes and cumulus cells. Therefore, to investigate the possible reproductiverelated implications of FGF9 supplementation, its effects on IVM oocytes after parthenogenetic activation (PA) using the pig model were examined. Five approaches were taken to explore the function of FGF9 during in vitro embryo production (IVP). First, the maturation medium was supplemented with exogenous FGF9 to investigate its effects on maturation of GV oocytes measured by the extrusion of the first polar body hence MII oocytes. The results showed that FGF9 significantly accelerated MII rates in 50 ng/ml treated group (p<0.05) but had no visible change on embryo development after PA. However, the addition of FGF9 in both maturation and embryo culture media improved MII rates at 50 ng/ml (p<0.05), cleavage at 100 ng/ml (p<0.05) and blastocysts rate significantly (p<0.05) across treated groups after PA. Third, the addition of FGF9 in embryo culture alone revealed no visible effect. Fourth, RNA sequence analysis showed that FGF9 significantly up-regulated Thyroid hormone (TRH) providing insight into its possible mechanisms of action during IVM. With regards to oocyte quality, mitochondria membrane potential, mitochondria number and ROS levels were significantly increased at 100 ng/ml (p<0.05), reduced at 50 ng/ml (p<0.05) and increased across all FGF9-treated groups (p<0.01 and <0.05) respectively. Taken together, the present study determined that at least another thecal- and oocyte-derived product, FGF9, improves oocyte maturation and subsequent embryo development when provided during IVM and IVC. These findings, therefore, may be useful for porcine IVP system. -
PHA処理がブタ体細胞核移植卵の体外発生能に及ぼす影響(Effect of phytohemagglutinin(PHA) on nuclear transfer pig embryos in vitro)
, ンドゥブイシ・マチェベ; 清水 聡一郎; 秦 仁樹; 大畠 一輝; 谷 哲弥; 加藤 容子 , Journal of Mammalian Ova Research , 36 , 1 , 33 , 43 , Apr. 2019
Summary:本研究では、ブタ単為発生卵ならびに体細胞核移植卵の体外発生能に及ぼすPHAの影響を検討した。単為発生卵ではPHA処理するとミトコンドリア活性が向上することがわかった。体細胞核移植卵では細胞融合前にPHAで5分間処理すると卵割率と胚盤胞への発生率が有意に向上した。また、ミトコンドリア関連遺伝子であるATPase6とND1の発現量が有意に増加した。以上の結果から、PHAの前処理は、ミトコンドリア活性に影響を及ぼしてブタ体細胞核移植卵の体外発生能を向上させることが示唆された。(著者抄録) - Pre-fusion treatment with phytohaemaglutinin-P (PHA-P) increases relative expression of mitochondria related genes in somatic cell nuclear transfer embryos in pig , Ndubuisi Samuel MACHEBE; Masaki HATA; Kohei ARIFUKU; Yurika NARUMIYA; Yuki ITANI; Kazuki OHATA; Tetsuya TANI; Yoko KATO , The 17th Asian-Australasian Association of Animal Production Societie , Aug. 2016
Patents
- オウレンおよびオウバク抽出物あるいはベルベリンまたはその塩を含有する避妊薬ならびにそれらを用いた受胎調節法 , 角田 幸雄, 加藤 容子
- 体細胞核初期化因子 , 角田 幸雄, 加藤 容子, 谷 哲弥 , 特許第3736517号
- 体細胞核初期化因子 , 角田 幸雄, 加藤 容子, 谷 哲弥
Research Grants & Projects
- 日本学術振興会, 科学研究費助成事業 基盤研究(C), 新規アッセイ系を用いた卵子のトランスクリプトーム解析 , 近畿大学
- JSPS外国人招聘研究者, (長期), ブタ卵子(胚)を用いた新しいポータブル培 養システムの開発
- JSPS外国人特別研究員事業, 科学研究費助成事業 特別研究員奨励費, ブタ幹細胞と初期化関連因子を用いたクローニングの効率化に関する研究 , 近畿大学